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论文题目: How understory vegetation affects the catalytic properties of soil extracellular hydrolases in a Chinese fir (Cunninghamia lanceolata) forest
第一作者: Yang Yang, Zhang Xinyu, Wang Huimin, Fu Xiaoli等
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发表年度: 2019
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英文摘要: To study how the understory vegetation influences the catalytic properties of extracellular hydrolases, we established a paired treatment experiment, with understory vegetation and litter removed from one treatment (understory removal, UR) and litter removed and the understory vegetation left intact in the other (control, CK), in a subtropical Chinese fir (Cunninghamia lanceolata) plantation. We used fluorescent substrates to determine the maximum velocity (V-max), the affinity of hydrolases to the substrates (K-m), and the catalytic efficiency (V-max/K-m) of three extracellular hydrolases (beta-1,4-glucosidase (beta G), beta-1,4-N-acetylglucosarninidase (NAG) and acid phosphatase (AP)). We found that the V-max, values for AG and NAG were 23.0% and 16.8% lower, respectively, in the UR treatment than in the CK treatment, and that the V-max for AP was similar in both treatments. The K-m and V-max/K-m for all the three hydrolases remained steady after the understory vegetation was removed. The soil C and N contents and the bacterial and fungal biomass were generally positively correlated with the V-max values. Thus, understory vegetation had more influence on the activities (V-max) than on the substrate affinities of extracellular hydrolases (K-m). Microbes tended to concentrate on maintaining the catalytic efficiency when the SOC contents were between 15.8 and 20.3 g kg(-1) when the understory vegetation was removed from a subtropical Chinese fir forest, and the catalytic efficiency could decrease under extremely low soil C contents. We suggest that understory vegetation should be maintained to sustain the potential microbial activity in subtropical Chinese fir forests.
刊物名称: EUROPEAN JOURNAL OF SOIL BIOLOGY
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论文类别: SCI
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